CONVENIENT METHOD OF ESTABLISHING PERMANENT LINES OF XERODERMA PIGMENTOSUM CELLS

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (2) , 253-256
Abstract
Nine lymphoblastoid cell lines were established after transformation by Epstein-Barr virus of peripheral lymphocytes from 4 xeroderma pigmentosum (XP) patients, the parents of 1 XP patient and 3 normal donors. All these cell lines proliferate as suspensions in Roswell Park Memorial Institute Medium 1640 supplemented with 20% fetal bovine serum, without detectable release of infectious Epstein-Barr virus. Some characteristics of these cell lines, such as growth rates, chromosome numbers, UV sensitivities and activities of unscheduled DNA syntheses induced by UV, 4-nitroquinoline-1-oxide and N-methyl-N''-nitro-N-nitrosoguanidine, were determined. Results confirm that the properties related to XP are not altered by transformation with Epstein-Barr virus and are the same in degrees of defect as those of dermal fibroblasts from the respective individuals. These XP and normal lymphoblastoid cell lines should be especially useful for biochemical studies on the mechanism of DNA repair, because they are easy to grow in mass culture.

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