Preservation of Encysted Polytomella

Publisher Website
Google Scholar
Abstract
Cysts of P. parva and P. caeca were recovered after 7 days storage at cryogenic temperatures following drying on shredded filter paper, silica gel or without added substrate. Accelerated storage testing, by exposing dried material to elevated temperatures, indicated that shredded filter paper was the best of the substrates tested. P. parva was recovered after 5 yr storage at -30.degree. C when dried on filter paper but not when dried on silica gel. Determinations of the number of cysts recovered indicated that viable cysts survived all conditions of storage tested. Excystment following storage was delayed, the extent depending on storage conditions and the substrate used for drying. Most rapid recovery occurred when cysts were rehydrated immediately after drying, and after storage on filter paper at below -70.degree. C.

This publication has 1 reference indexed in Scilit: