Redox-dependent downregulation of Rho by Rac
Top Cited Papers
- 24 February 2003
- journal article
- letter
- Published by Springer Nature in Nature Cell Biology
- Vol. 5 (3) , 236-241
- https://doi.org/10.1038/ncb938
Abstract
Rac and Rho GTPases function as critical regulators of actin cytoskeleton remodelling during cell spreading and migration. Here we demonstrate that Rac-mediated reactive oxygen species (ROS) production results in the downregulation of Rho activity. The redox-dependent decrease in Rho activity is required for Rac-induced formation of membrane ruffles and integrin-mediated cell spreading. The pathway linking generation of ROS to downregulation of Rho involves inhibition of the low-molecular-weight protein tyrosine phosphatase (LMW-PTP) and then an increase in the tyrosine phosphorylation and activation of its target, p190Rho-GAP. Our findings define a novel mechanism for the coupling of changes in cellular redox state to the control of actin cytoskeleton rearrangements by Rho GTPases.Keywords
This publication has 32 references indexed in Scilit:
- Rho GTPases in cell biologyNature, 2002
- Two Vicinal Cysteines Confer a Peculiar Redox Regulation to Low Molecular Weight Protein Tyrosine Phosphatase in Response to Platelet-derived Growth Factor Receptor StimulationPublished by Elsevier ,2001
- Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3Current Biology, 2000
- The Low M r Protein-tyrosine Phosphatase Is Involved in Rho-mediated Cytoskeleton Rearrangement after Integrin and Platelet-derived Growth Factor StimulationPublished by Elsevier ,2000
- Rac Downregulates Rho ActivityThe Journal of cell biology, 1999
- Inhibition of Myosin Light Chain Kinase by p21-Activated KinaseScience, 1999
- The Inactivation Mechanism of Low Molecular Weight Phosphotyrosine-protein Phosphatase by H2O2Journal of Biological Chemistry, 1998
- The Guanine Nucleotide Exchange Factor Tiam1 Affects Neuronal Morphology; Opposing Roles for the Small GTPases Rac and RhoThe Journal of cell biology, 1997
- Rac “Insert Region” Is a Novel Effector Region That Is Implicated in the Activation of NADPH Oxidase, but Not PAK65Published by Elsevier ,1996
- PDGF receptor as a specific in vivo target for low Mr phosphotyrosine protein phosphataseFEBS Letters, 1995