On the Metabolic Alteration of Pteroylglutamic Acid.

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Abstract
The formation of citrovorum factor (CF) from pteroylglutamic acid (PGA) was studied in vitro in incubating tissues slices and homogenates. The liver appears to be the main site of this transformation. Other tissues had very limited capacity to form CF, with the exception of bone marrow which, relative to the small amt. present in this tissue, formed an appreciable quantity of the factor. The hepatic tissue of rats which had been depleted of folic acid and CF was flooded with CF within one hr. following the intraperitoneal injn. of PGA. Homogenates of rat or chick liver, when incubated with PGA under an atmosphere of N, were capable of forming CF. Little CF was found when similar prepns. were incubated under O2. The yield of CF was consistently increased by the presence of ascorbate in the incubation mixture.