Detection of 2‐Amino‐1‐methyl‐6‐(4‐hydroxyphenyl)imidazo[4,5‐b]pyridine in Broiled Beef
Open Access
- 1 September 1992
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 83 (9) , 919-922
- https://doi.org/10.1111/j.1349-7006.1992.tb02000.x
Abstract
2‐Amino‐1‐methyl‐6‐(4‐hydroxyphenyl)imidazo[4,5‐b]pyridine (4′‐OH‐PhIP) showed mutagenicity in Salmonella typhimurium TA98 in the presence of S9 mix, inducing 180 revertants per 100 μg. Since creatinine, tyrosine and glucose, which are present in meat, are expected to be involved in the formation of 4′‐OH‐PhIP, its presence in broiled beef was examined. 4′‐OH‐PhIP was detected in broiled beef by high‐performance liquid chromatography and UV‐spectrometry after extraction with 0.1 N HCl and purification by blue cotton treatment and ion exchange column chromatography. Its level was estimated to be 21.0 ng per g of broiled beef, which is comparable to that of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP).Keywords
This publication has 21 references indexed in Scilit:
- Induction of Intestinal Adenocarcinomas by 2‐Amino‐l‐methyl‐6‐phenylimidazo‐[4,5‐b] pyridine in Nagase Analbuminemic RatsJapanese Journal of Cancer Research, 1991
- Metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP) by liver microsomes and isolated rabbit cytochrome P450 isozymesCarcinogenesis: Integrative Cancer Research, 1990
- Differential rates of metabolic activation and detoxication of the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine by different cytochrome P450 enzymesCarcinogenesis: Integrative Cancer Research, 1990
- Induction of Lymphoma in CDF1 Mice by the Food Mutagen, 2‐Amino‐l‐methyl‐6‐phenylimidazo[4,5‐b]pyridineJapanese Journal of Cancer Research, 1989
- Carcinogenic tryptophan pyrolysis products in airborne particles and rain waterMutation Research Letters, 1989
- 4-(2-amino-1-methylimidazo pyrid-6-yl)[4,5-b]phenyl sulfate—a major metabolite of the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b] (PhIP) in the ratCarcinogenesis: Integrative Cancer Research, 1989
- Formation of a mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine (PhIP) in cooked beef, by heating a mixture containing creatinine, phenylalanine and glucoseMutation Research Letters, 1987
- Studies on Environmental Chemical Carcinogenesis in JapanScience, 1986
- Qualitative and quantitative characterization of mutagens in commercial beef extractCarcinogenesis: Integrative Cancer Research, 1983