Regulation of Nervous System‐Specific S‐100 Protein and Enolase Levels in Adipose Tissue by Catecholamines

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Abstract
The effect of catecholamines on the levels of S-100 protein and nervous system-specific enolase (NSE) in epididymal adipose tissue of Wistar rats in vivo was examined by sensitive enzyme immunoassay methods. Soluble S-100 protein levels in the adipose tissue of 9-12-week-old rats (1.46 .+-. 0.19 .mu.g/mg protein) were decreased to < 50% of those of controls by serial injection (for 4-7 days) of epinephrine (0.1 mg/day) or norepinephrine (0.15 mg) with, however, little effect on the levels of membrane-bound (pentanol-extractable) S-100 protein. A significant decrease in the soluble S-100 protein levels was observed at 2 h after a single injection of epinephrine (1.04 .+-. 0.13 .mu.g/mg protein). Levels of NSE subunit (.gamma. subunit or 14-3-2 protein) in adipose tissue (0.51 .+-. 0.03 .gamma..gamma.-equivalent pmol/mg protein) were increased to 170% of control by serial injection (for 7 days) of epinephrine or norepinephrine with little change of the level of enolase .alpha. subunit on a mg protein basis. Isoproterenol had no apparent effect on the levels of soluble S-100 protein and NSE subunit. The levels of S-100 protein and NSE in adipose tissue are regulated by catecholamines.