Prevention of Ozonide-Induced Heinz Bodies in Human Erythrocytes by Vitamin E

Abstract
Methyl oleate ozonide, a proposed intermediary in ozone intoxication, produced Heinz body inclusions in human erythrocytes at concentrations of from 10−4 to 2×10−3 M. Daily oral supplementation with either 100 mg or 200 mg of d-α- tocopheryl acetate prevented Heinz body formation by methyl oleate ozonide. These observations suggest that the protective effects of vitamin E against ozone-produced toxicants occurs in man as well as in animals.

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