The role of glutathione in renal cortical tissue. effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 18 (2-3) , 109-115
- https://doi.org/10.1007/bf00280276
Abstract
The effects of diamide were studied in rat kidney cortical tissue. It was found that diamide increased oxidized glutathione levels and inhibited Na+-K+-ATPase activity. Consistent with this finding was the observation that diamide compromised the sodium gradients maintained in renal cortical slices. Amino acid transport studies with ouabain or a sodium-free buffer indicated that diamide interferes with both Na+-dependent and Na+-independent transport systems. These results indicate that diamide has a number of different effects on renal cortical tissue and emphasize the important role of glutathione in maintaining control of a number of key metabolic pathways.Keywords
This publication has 42 references indexed in Scilit:
- Reversible inhibition by diamide of cyclic AMP-dependent protein kinases from bovine thyroidBiochemical and Biophysical Research Communications, 1977
- Reconstitution of specific Na+-dependent D-glucose transport in liposomes by Triton X-100 extracted proteins from purified brush border membranes of hamster small intestineBiochemical and Biophysical Research Communications, 1976
- Non-linearity of human renal (Na+ + K+)-ATPase: Elimination by purification and correlation with cyclic AMP-dependent protein kinase activityBiochemical and Biophysical Research Communications, 1976
- Sugar transport by renal plasma membrane vesiclesThe Journal of Membrane Biology, 1975
- Specific inhibition of protein kinase by diazenedicarboxylic acid bis-(N,N-dimethylamide)Biochemical and Biophysical Research Communications, 1975
- Action of cytochalasin A, A sulfhydryl-reactive agent, on sugar metabolism and membrane-bound adenosine triphosphatase of yeastBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- The effect of diamide and glutathione on the uptake of α-methyl-d-glucoside by slices of rat kidney cortexBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Phosphorylation and dephosphorylation of renal brush border membranes by protein kinase and phosphoprotein phosphataseFEBS Letters, 1975
- The inhibition of ribonucleic acid synthesis by the thiol‐oxidizing agent, diamide, in Escherichia coliFEBS Letters, 1970
- Diamide, a new reagent for the intracellular oxidation of glutathione to the disulfideBiochemical and Biophysical Research Communications, 1969