Antisense Oligodeoxynucleotides for IL‐2, c‐myc and Transferrin Receptor Synchronize Mitogen‐Activated Lymphocytes in the G1 Phase

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Abstract
In order to clarify the role and interrelationship of c‐myc, interleukin‐2 (IL‐2), and transferrin receptor (TfR) expressions on PHA‐stimulated lymphocyte prohferation, we examined the effects of antisense oligodeoxynucleotides directed against c‐myc, TfR, and IL‐2 mRNAs on DNA synthesis and cell‐cycle phase. Exposure of PHA‐stimulated lymphocytes to each antisense oligomer resulted in approximately 75 80% inhibition of DNA synthesis. TfR expression was not inhibited in PHA‐stimulated lymphocytes by c‐myc or IL‐2 antisense oligonucleotides, suggesting that the expression of c‐myc, TfR, and of IL‐2 is regulated by an independent mechanism. All three antisense oligonucleotides for c‐myc, TfR and IL‐2 synchronized mitogen‐activated lymphocytes to the G1 phase as assessed by morphologic blast formation and cell‐cycle phase analysis.