Factors Influencing the in Vitro Cultivation of Common Bean (Phaseolus Vulgaris L.) Leaf Petioles

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Abstract
The influence of genotype, explant's age and medium on in vitro cultivation of common bean (Phaseolus vulgaris L.) leaf petioles was investigated. Bulgarian cultivars Doubru-djanski 7; Plovdiv 11M and Plovdiv 10 were used in the experiments. Leaf petiole explants arising from in vitro seedlings were cultivated on modified MS media containing different amounts of TDZ, Paclobutrazol, NAA, BAP, IBA and GA3 in different combinations. Explant's development in different ages (5-; 7- and 14-days old) on the investigated media was genotype dependent. Bi-factorial ANOVA and three-factorial analyses were used for evaluation of the results that precisely disclosed the factor's interactions. The applied 3-factorial dispersion analysis revealed that the interaction between factors genotype-explant age (AB) and the threefold interaction genotype-explant age-media (ABC) exerted the strongest influence on the explant development and callus formation. The influence of interaction between factors genotype-explant's age (AB) increase along with cultivation time. No dependence was found between the size of initial plants, leaf petiole position (near to the leaf or to the steam), explant fresh weight, their development and quantity of formed callus. An additional experiment with precultivated sterile seeds on media containing 1 μM IAA or 1 μM BAP (on the base of MS) contributed. Better explant development was observed in comparison to their cultivation only on MS medium.