Studies on hydrogen-utilizing microorganisms. V. Isolation and culture conditions of thermophilic hydrogen bacteria.

1 January 1977

journal article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 41 (4) , 685-690
https://doi.org/10.1271/bbb1961.41.685

Abstract

Isolation of thermophilic hydrogen bacteria was performed at 50°C using enrichment culture method. One of the four strains isolated, strain TH-1 grew most rapidly. Culture conditions of strain TH-1 were investigated. Optimum temperature and pH for growth proved to be 52°C and 7.0, respectively. There existed a positive correlation between the specific growth rate and the partial pressure of carbon dioxide in the gas phase. Ammonium and nitrate are the good nitrogen sources in that order. Effect of concentrations of nitrogen source, magnesium, ferrous and phosphate ions on the cell growth was also investigated. The maximum specific growth rate (μ_max) of strain TH-1 was determined as 0.68 hr^-1 by the cultivation at 52°C in a jar fermentor containing the optimal medium at pH 7.0.

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