Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines.
- 1 July 1999
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 80 (7) , 1725-1733
- https://doi.org/10.1099/0022-1317-80-7-1725
Abstract
The complete nucleotide sequences of the human papillomavirus type 16 (HPV-16) variants present in the CaSki and SiHa cervical carcinoma cell lines and the primary subgenomic HPV-18 variant present in the HeLa cervical carcinoma cell line were determined using overlapping bulk PCR products as templates. PCR-based methods were also used to characterize five previously unreported CaSki HPV-16 genomic disruptions and the 5' cellular-viral junction common to all HeLa HPV-18 subgenomic structures.Keywords
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