Feulgen-Deoxyribonucleic Acid Values in the Cytology of Bladder Cancer

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Abstract
The DNA values of cells separated from fixed specimens from patients with bladder cancer were determined with the use of a microspectrophotometer and were compared to the grade and stage of cancer tissues. A histogram of nuclear DNA values showed flatness of the mode, deviation of the stem line to the right and an increase of polyploid cells associated with an increase in the grade and stage of cancer. Prognosis seemed related more closely to DNA values of cancer tissues than to the stage and grade of cancer. DNA values of exfoliated cells from fresh cancer tissue and the cells in the urine of the same patients were compared, revealing similar patterns. Indication of grade was made in some cases by determining nuclear DNA values of exfoliated cells in urine. Determination of nuclear DNA values apparently is a valuable parameter for automated evaluation of urinary cytology.