Molecular cloning and characterization of the human p27Kip1 gene promoter1
Open Access
- 7 July 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 411 (1) , 1-6
- https://doi.org/10.1016/s0014-5793(97)00660-1
Abstract
P27Kip1 is an inhibitor of multiple cyclin‐dependent kinases (cdk), and can arrest the cell‐cycle progression by inhibiting the phosphorylation of the retinoblastoma gene family products. Tumor formation in p27Kip1 knockout mice clearly shows that p27Kip1 plays an important role in inhibiting tumor formation and progression. To investigate the mechanism of transcriptional p27Kip1 gene expression, we isolated the genomic DNA fragment of the 5′ flanking region of the human p27Kip1 gene and characterized its promoter region. The human p27Kip1 promoter is TATA‐less, and the sequence is highly homologous to the murine p27Kip1 promoter sequence. In the promoter assay, deletion from −774 to −435 relative to the initiating codon resulted in a 15–20‐fold reduction of the p27Kip1 promoter activity, suggesting that the elements for basal promoter activity exist in this highly conserved 340 bp region, where putative CTF and ATF sites are conserved.Keywords
This publication has 27 references indexed in Scilit:
- Inhibitors of mammalian G1 cyclin-dependent kinases.Genes & Development, 1995
- Novel INK4 Proteins, p19 and p18, Are Specific Inhibitors of the Cyclin D-Dependent Kinases CDK4 and CDK6Molecular and Cellular Biology, 1995
- p57KIP2, a structurally distinct member of the p21CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene.Genes & Development, 1995
- Cyclic AMP-induced G1 phase arrest mediated by an inhibitor (p27Kip1) of cyclin-dependent kinase 4 activationCell, 1994
- pl5INK4B is a potentia| effector of TGF-β-induced cell cycle arrestNature, 1994
- p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21Published by Elsevier ,1994
- Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signalsCell, 1994
- p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.Genes & Development, 1994
- A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4Nature, 1993
- The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinasesCell, 1993