Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.
Open Access
- 1 December 1986
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 103 (6) , 2103-2112
- https://doi.org/10.1083/jcb.103.6.2103
Abstract
Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of yeast cells that overproduce the RNA2 and RNA3 proteins has revealed that these proteins are enriched in nuclear fractions. Indirect immunofluorescence results have indicated that these proteins are localized in yeast nuclei. These localization results are consistent with the fact that these genes have a role in processing yeast pre-mRNA.This publication has 48 references indexed in Scilit:
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