Lipid-Mediated Light Activation of a Mechanosensitive Channel of Large Conductance
- 16 July 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 20 (17) , 6985-6987
- https://doi.org/10.1021/la048942v
Abstract
This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-{[4-(4-butylphenyl)azo]phenoxy}pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL.Keywords
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