THIAMINE-INDUCED ALTERATION IN STEROL COMPOSITION OF SACCHAROMYCES-CARLSBERGENSIS-4228

  • 1 January 1978
    • journal article
    • research article
    • Vol. 531  (1) , 86-95
Abstract
The cells of S. carlsbergensis 4228 grown with added thiamine in a vitamin B-6-free medium did not contain ergosterol and zymosterol (the predominant sterols in cells grown without thiamine). Instead, significant amounts of squalene, lanosterol and unidentified sterols accumulated in the thiamine-grown cells. Of the unidentified sterols, the most predominant was .DELTA.5,7-ergostadien-3.beta.-ol at 26.0% of the total sterol. The accumulation of this sterol in the place of ergosterol (.DELTA.5,7,22-ergostatrien-3.beta.-ol) indicates that the desaturation at C-22 of sterol structure is completely blocked in the thiamine-grown cells. On the basis of chromatographic behaviors, the structures of other unidentified sterols were assumed to be 4.alpha.-methyl-.DELTA.8,24(25)-cholestadien-3.beta.-ol, 4.alpha.-methyl-.DELTA.8,24(28)-ergostadien-3.beta.-ol, .DELTA.8,24(28)-ergostadien-3.beta.-ol and .DELTA.5,7,24(28)-ergostatrien-3.beta.-ol. The accumulation of 4.alpha.-methyl sterols, in addition to that of a large amount of lanosterol (48.4% of total sterol), suggests that the demethylation processes from lanosterol to zymosterol was partially depressed in the thiamine-grown cells.

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