Role of Extended Culture Time on Synergy of Fluconazole and Human Monocyte-Derived Macrophages in Clearing Candida albicans

Abstract
Possible enhanced candidacidal synergy of fluconazole and human monocyte-derived macrophages by extended culture time was studied. Synergistic candidacidal activity increased from 1 day to 3 days and was maximal at 6 days. Enhanced synergistic candidacidal activity at 144 h was observed over a l0-fold challenge dose range. Enhanced synergy with culture time was seen with fluconazole-susceptible and -resistant isolates of Candida albicans. These findings emphasize the importance of dosage and an adequate period of therapy.

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