EVIDENCE FOR THE EXISTENCE OF MULTIPLE FORMS OF CHOLINE (ETHANOLAMINE) KINASE IN RAT-TISSUES

  • 1 January 1985
    • journal article
    • research article
    • Vol. 833  (1) , 1-8
Abstract
In order to characterize the form of choline kinase in rat tissues, both electrophoretic and gel chromatographic patterns of choline kinase activity were compared in the liver, kidney, lung, whole intestine and carbon tetrachloride-induced liver cytosols. Kinetic parameters of the reaction were also compared for the main forms of choline kinase protein from these tissues. The overall results suggested strongly that choline kinase does not exist in 1 particular active form but exists in multiple forms in rat tissues. The electrophoretic patterns of both choline kinase and ethanolamine kinase activities were compared in rat liver, kidney, lung and intestinal cytosols. Results supported the view that both kinase activities are represented on the same enzyme protein(s) in each of the rat tissues examined.