Large‐scale isolation of equine liver alcohol dehydrogenase on a blue agarose gel
- 1 May 1979
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 21 (5) , 775-785
- https://doi.org/10.1002/bit.260210504
Abstract
Equine liver alcohol dehydrogenase (EC 1.1.1.1) has been purified by a new scheme using a blue agarose gel (Blue Sepharose) as an affinity sorbent. Starting amounts of 0.6 to 10 kg liver have been processed to enzyme possessing 1.5 U/mg average specific activity, in about three to four days. Some parameters concerning adsorption of enzyme to the blue gel as well as recovery therefrom have been explored.This publication has 10 references indexed in Scilit:
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