EFFECT OF HYDROXYUREA AND VINBLASTINE ON PROLIFERATION OF PLURIPOTENTIAL STEM-CELLS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (1) , 29-40
Abstract
The population of pluripotential hemopoietic stem cells in mice, i.e., those cells forming colonies in spleens of lethally irradiated mice (colony forming cells [CFc] proliferates relatively slowly. After partial damage the population regenerates by increased proliferation rates. The effect of damage caused by different doses of the antitumor agents, hydroxyurea or vinblastine, on the proliferation of the CFc was investigated. CFc population was measured in femur bone marrow after grafting the sample of the bone marrow into lethally irradiated recipients (spleen colony method). The proliferation rate was estimated according to the magnitude of the fraction of cells synthesizing DNA in the S phase of the cell cycle, or according to the sensitivity of the population to repeated injections of vinblastine. Even after minute damage caused by hydroxyurea the stem cells proliferated intensively. The effect was dose dependent. Comparable damage caused by vinblastine had a significantly weaker effect on the proliferation of stem cells. The proliferation response of the pluripotential stem cells depends on 2 factors: the extent of damage to hemopoietic tissue and the position of killed cells in the cell cycle.

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