Phycocyanin synthesis and degradation in the blue-green bacterium Anacystis nidulans

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Abstract
Cellular content and rates of synthesis of the apoprotein subunits of phycocyanin in A. nidulans cultures undergoing, and recovering from, nitrate starvation were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total and immunoprecipitable soluble proteins. Results indicated that nitrate starvation provokes coordinate degradation of apoprotein subunits; de novo synthesis of these subunits is selectively depressed during starvation; nitrate restoration provokes coordinate increases in the rates of synthesis of these subunits, although maximal rates are not achieved for 6-10 h after readdition of nitrate; and illumination affects both relative and absolute rates of apoprotein formation.