Fundamental Studies on Clinical Chemistry.XI.A New Micromethod for the Determination of General Ketosteroids using p-Nitrophenylhydrazine Reagent

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Abstract
A highly sensitive spectrophotometric method for the determination of general ketosteroids was investigated. The development of chromophore with p-nitrophenylhydrazine reagent was achieved efficiently in alkaline dimethylformamide. Micro amounts (0∼50 μg. for saturated ketosteroids ; 0∼25 μg. for Δ4-3-ones) of steroids were determined by the standard procedure. One of the remarkable advantages is the possibility to estimate micro quantities of the saturated 3-ketosteroids such as cholestan-3-one, that has been almost impossible by the most published methods. A discussion on the reactivities of different kinds of steroidal keto group for the method derived was also presented from the results of the study using forty oxosteroids.

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