Effect of dibutyryl cyclic AMP on morphologic features and biologic markers of a human salivary gland adenocarcinoma cell line in culture

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Abstract
Dibutyryl cyclic AMP (dB‐cAMP) has marked effects on the growth, morphologic features, and biologic markers of a human salivary gland adenocarcinoma cell line in culture. A cell line with ultrastructure and biologic markers specific to the intercalated duct cells of human salivary glands was cultivated in the presence of dB‐cAMP. Major alterations, such as process formation and expression of myofilaments and oxytocin receptor in addition to myosin and S‐100 protein, were observed in those cells with a phenotype similar to myoepithelial cells. Both the anchorage‐independent and anchorage‐dependent growths were markedly suppressed in the presence of dB‐cAMP. After the removal of dB‐cAMP from the culture, the treated cells returned rapidly to the phenotype of the untreated cells. These findings indicate that reversible differentiation into the myoepithelial cells of a human salivary gland adenocarcinoma cell line occurs in growth medium containing dB‐cAMP.

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