Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 66 (1-3) , 67-73
- https://doi.org/10.1016/0378-1097(90)90260-w
Abstract
A fragment of chromosomal DNA from Proteus vulgaris encoding urease was cloned and expressed in Escherchia coli. A 3 kbp region was sequenced and revealed three open reading frames with homology to jack (Canavalia ensiformis) urease. The smallest protein (11 kDa) was homologous to the N-terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C-terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease.Keywords
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