ALTERATIONS IN PROTEIN AND NUCLEIC ACID METABOLISM OF LYMPHOMA 6C3HED-OG CELLS IN MICE GIVEN GUINEA PIG SERUM

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Abstract
Lymphoma 6C3HED-OG cells, known from previous work to be susceptible to the effects of guinea pig serum in vivo and dependent upon extrinsic asparagine for protein synthesis and growth in vitro, remained for the most part morphologically intact and countable in the electronic cell counter following exposures of 1 and 2 hr to the effects of heated (56°C, 30 min) guinea pig serum injected into the peritoneal cavities of mice in which the lymphoma cells were growing rapidly; after exposures of 4 and 6 hr the bulk of the -OG cells remained still intact and countable in the cell counter, though by this time a small proportion of them (5 to 12%) proved stainable with eosin in wet preparations) hence were presumably nonviable.