In VitroEvolution of Proteins for Drug Development
- 1 February 2003
- journal article
- review article
- Published by Mary Ann Liebert Inc in ASSAY and Drug Development Technologies
- Vol. 1 (supplement) , 187-198
- https://doi.org/10.1089/154065803321537917
Abstract
Thanks to biotechnology, proteins are becoming increasingly important tools to fight disease, both as therapeutics in their own right and as catalysts for the synthesis of small molecule drugs. However, the properties of these proteins are not necessarily optimal for their intended tasks. In vitro evolution is a set of technologies useful to address their shortcomings. Moreover, in vitro evolution can help illuminate natural evolutionary pathways, thus potentially enabling prediction of drug resistance evolution. We consider here recent developments in the area of in vitro evolution, as well as its application to proteins of interest to medical science.Keywords
This publication has 70 references indexed in Scilit:
- Integrin αvβ3‐targeted therapy for Kaposi's sarcoma with an in vitro‐evolved antibodyThe FASEB Journal, 2002
- Evolution of an Antibiotic Resistance Enzyme Constrained by Stability and Activity Trade-offsJournal of Molecular Biology, 2002
- Correction: retraction: Directed evolution of new catalytic activity using the α/β-barrel scaffoldNature, 2002
- Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAsNature Biotechnology, 2002
- A Novel Strategy by the Action of Ricin that Connects Phenotype and Genotype without Loss of the Diversity of LibrariesJournal of the American Chemical Society, 2002
- Predicting the emergence of resistance to antifungal drugsFEMS Microbiology Letters, 2001
- Directed evolution of a novel N-carbamylase/D-hydantoinase fusion enzyme for functional expression with enhanced stabilityBiotechnology & Bioengineering, 2000
- Regional Codon Randomization: Defining a TATA-Binding Protein Surface Required for RNA Polymerase III TranscriptionScience, 1993
- Phage antibodies: filamentous phage displaying antibody variable domainsNature, 1990
- Amplification, mutation and selection of catalytic RNAGene, 1989