Localization of human type II procollagen gene (COL2A1) to chromosome 12
- 1 November 1984
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 10 (6) , 651-655
- https://doi.org/10.1007/bf01535232
Abstract
DNA was prepared from 39 humanmouse somatic cell hybrid lines, and mouse and human parental cell lines. The DNA was digested to completion with EcoRI and Southern filters were prepared. These filters were hybridized at high stringency conditions to the human genomic subclone phHCo1 (II)A which corresponds to the human α1 (type II) procollagen gene (COL2A1). The mouse DNA yielded a single band at >10 kb, whereas the human DNA had the expected single band at 4.8kb. Analyses of these human-mouse cell hybrids demonstrated that the human α1(type II) procollagen gene segregates with chromosome 12.Keywords
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