Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. II. Specificity of the Method in Pyridoxal-5-phosphate Determination.
- 1 January 1966
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 14 (5) , 488-491
- https://doi.org/10.1248/cpb.14.488
Abstract
The specificity of the previously reported fluorometric method of determination of pyrldoxal-5-phosphate (PALP) in biological materials was investigated. The fluorescence excitation and emission spectra, the change in fluorescence Intensity with pH, the electrophoresis, the paper chromatography, and the hydrolysis with acid phosphatase were examined with the PALP fraction obtained from mouse liver. The results showed that the method had high specificity for the determination of PALP in liver.This publication has 3 references indexed in Scilit:
- Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. I. Procedure and Application.CHEMICAL & PHARMACEUTICAL BULLETIN, 1966
- The reaction of pyridoxal 5-phosphate with cyanide and its analytical useArchives of Biochemistry and Biophysics, 1960
- CHOLINE PHOSPHOKINASE1953