Contact sensitivity in the mouse. XII. The use of DNA synthesis in vivo to determine the anatomical location of immunological unresponsiveness to picryl chloride.

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  • 1 September 1973
    • journal article
    • Vol. 25  (3) , 495-508
Abstract
[125I]iododeoxyuridine was used to assess the DNA synthesis in the lymph nodes and spleen of mice exposed to picrylating agents. Skin painting with picryl chloride causes both contact (delayed) hypersensitivity and antibody production. It also increases DNA synthesis in the draining lymph nodes and spleen. The lymph node response peaks at day 3 and reverts to normal by day 7. In contrast, the DNA synthesis in the spleen shows three well-defined peaks on days 2, 5 and 8. The total DNA synthesis in the first 4 days is 0.6–2.4 times greater in the spleen than in the draining lymph nodes.