Studies on Encystment of Echinostoma revolutum Cercariae

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Abstract
Cercariae of E. revolutum encysted in the kidney of the snail Physa heterostropha within 1 h and on mucus trails from Helisoma trivolvis, P. heterostropha and Lymnaea sp. within 2 h. Significantly, more normal cysts were formed in mucus of Helisoma than in mucus of Physa or Lymnaea. Optimal, in vitro encystment occurred within 24 h in either Locke''s 1:1 or Locke''s 1:1 + 1% glucose. Significantly more normal cysts occurred in the Locke''s 1:1 medium. Both normal and abnormal cysts from Locke''s media and snail mucus excysted in an alkaline bile-trypsin medium. Cercariae did not encyst in Locke''s media supplemented with casein hydrolysate or in agar cultures containing various chemicals.