Effect of microtubule inhibitors on the tubulin system ofDictyostelium discoideum
- 1 January 1982
- journal article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 22 (2) , 127-131
- https://doi.org/10.1002/jobm.3630220208
Abstract
Indirect immunofluorescence with antibodies to microtubular proteins has been used to investigate the microtubule system of Dictyostelium discoideum vegetative amoebae and the action of several compounds that interfere with that system. All the inhibitors tested show an antimicrotubular effect. Colchicine, vinblastine, nocodazole, thiabendazole, and isopropyl-N-phenyl-carbamate (IPC) seem to act mainly by destroying microtubules leaving the appearance of nuclei associated organelles (NAOs) undisturbed. On the other hand griseofulvin also affects NAOs which disappear in treated cells.Keywords
This publication has 10 references indexed in Scilit:
- Immunofluorescence of the tubulin system in cellular slime mouldsFEMS Microbiology Letters, 1979
- Decoration and stabilization of intact, smooth-walled microtubules with microtubule-associated proteinsBiochemistry, 1979
- Characterization of colchicine-binding activity in Dictyostelium discoideumBiochemical Journal, 1978
- MicrotubulesPublished by Springer Nature ,1978
- Ultrastrukturelle Untersuchungen zur antimitotischen Aktivität von Methylbenzimidazol-2-ylcarbamat (MBC) und seinen Einfluß auf die Replikation des Kern-assoziierten Organells (“centriolar plaque”, “MTOC”, “KCE”) beiAspergillus nidulansProtoplasma, 1977
- Structural and biochemical aspects of cell motility in amebas of Dictyostelium discoideum.The Journal of cell biology, 1977
- The effect of inhibitors of microtubule and microfilament function on the cellular slime mould Dictyostelium discoideumExperimental Cell Research, 1976
- Microtubule formation from two components separated by gel filtration of a tubulin preparationBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- 3H-colchicine binding: Failure to detect any binding to soluble proteins from various lower organismsExperimental Cell Research, 1973
- Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic cultureBiochemical Journal, 1970