45-KDa GTP-binding Protein from Rat Olfactory Epithelium: Purification, Characterization and Localization
Open Access
- 1 April 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Chemical Senses
- Vol. 21 (2) , 181-188
- https://doi.org/10.1093/chemse/21.2.181
Abstract
The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with α-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed. Chem, Senses 21: 181–188, 1996.Keywords
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