ROLE OF PLATELETS IN LYSIS OF DILUTE PLASMA CLOTS - REQUIREMENT FOR METABOLICALLY ACTIVE PLATELETS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 94  (2) , 285-294
Abstract
The role of [human] platelets in clot lysis was investigated functionally with the use of dPRP [dilute platelet rich plasma] clots formed at 4.degree. C knd shifted to 37.degree. C. Clots handled in this manner lysed in 6 h (.+-. 1 h), whereas clots formed at 4.degree. C or at 37.degree. C and held at those temperatures, or clots formed from dPPP (dilute platelet poor plasma) did not lyse in less than 20 h. dPRP clots having the shorter (6 h) lysis time released 14C-5-HT [5-hydroxytryptamine-14C-creatinine sulfate] at the time of the temperature shift. Preincubation of dPRP with antimycin A and 2-deoxy-D-glucose before addition of thrombin prolonged the clot lysis to 26 h and inhibited release of 14C-5-HT at the time of the temperature shift. Metabolically active platelets were required to mediate the optimal clot lysis seen in the 4.degree. C to 37.degree. C system and they continued to function (i.e., take up and release 5-HT) after they were incorporated into a clot. The dPRP clot lysis system provided a model for studying the timing and sequence of the interaction of metabolically active platelets with the fibrin framework of the formed clot.