Lon-dependent regulation of the DNA binding protein HU in Escherichia coli.
- 1 October 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (20) , 7691-7695
- https://doi.org/10.1073/pnas.86.20.7691
Abstract
HU, the major DNA binding protein of Escherichia coli, exists in solution as a heterodimer composed of two highly homologous subunits: HU1, encoded by hupB; HU2, encoded by hupA. The purification of the HU protein from hupA- or hupB- bacteria showed that the hupB mutant strains synthesize normal amounts of the HU2 subunit (which corresponds to 60% of the total HU present in wild-type cells). On the contrary, the amount of HU1 present in hupA mutant strains corresponds to only 6% of the total HU present in wild-type cells. We showed by fusions of the hupB and hupA promoters to the malPQ operon that the absence of one subunit has no major effect on the transcription rate of the gene encoding the other subunit. Analysis of the stability of the HU1 and HU2 subunits, using pulse-chase labeling experiments, showed that the HU1 subunit is degraded specifically in the absence of the HU2 subunit and, moreover, that this degradation is dependent on the presence of the Lon protease.This publication has 28 references indexed in Scilit:
- Interaction of the Escherichia coli HU protein with DNAJournal of Molecular Biology, 1986
- A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: protein and DNA substrate requirements.Proceedings of the National Academy of Sciences, 1985
- Affinity of protein HU for different nucleic acidsFEBS Letters, 1985
- Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genesCell, 1985
- A convenient technique to compare the efficiency of promoters inEscherichia coliNucleic Acids Research, 1985
- Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- Native Escherichia coli HU protein is a heterotypic dimerFEBS Letters, 1979
- E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNACell, 1979