Isolation and characterization of cinobufagin 3-glutaroyl-L-arginine ester from Bufo bufo gargarizans Cantor.

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Abstract
A new bufotoxin having glutaric acid as a dicarboxylic acid moiety was found in the Korean toad, Bufo bufo gargarizans CANTOR. This new bufotoxin was separated from the dried toad by usual chromatographic methods including reversed phase high-performance liquid chromatography, and its structure was elucidated to be cinobufagin 3-glutaroyl-L-arginine ester by degradative and synthetic means. In addition, seven known bufogenins and eight known bufotoxins were isolated and characterized.

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