High-Pressure Liquid Chromatographic Assay of Azlocillin and Mezlocillin with an Anion-Exchange Extraction Technique

Abstract
An anion-exchange column deproteination technique has been employed with the high-pressure liquid chromatographic (LC) assay for azlocillin and mezlocillin. The anionexchange extraction gave excellent (97%-99%) drug recovery. Quantitation of antibiotics using the LC method compares favorably to the traditional biological assay technique with correlation coefficients for azlocillin = 0.998 and mezlocillin = 0.988. The anion-exchange extraction provides an interference-free chromatogram which aids in the LC assay of these drugs.