Sensitive Determination of Zearalenone and α-Zearalenol in Barley and Job's-Tears by Liquid Chromatography with Fluorescence Detection

Open Access
Publisher Website
Google Scholar
Abstract
A sensitive and reliable method for liquid chromatographic (LC) determination of zearalenone and α-azearalenol in barley and Job's-tears was investigated. The method by which these toxins were determined involves addition of an internal standard (zearalenone 6'-oxime) to barley and Job's-tears samples. Extracts from grain samples were cleaned up by passage through chromatography on piperidinohydroxypropyl Sephadex LH-20 as a lipophilic gel. Individual toxins were resolved by LC on a reversed-phase (ODS) column with fluorescence detection. The detection limit is estimated to be 0.2 ng for zearalenone and α-zearalenol standards. Known amounts of zearalenone and α-azearalenol (25-1250 ng) were added to a barley sample (5 g). Average recoveries for α-zearalenol and zearalenone, respectively, ranged from 96 to 102% (mean CV, 3.6%) and from 96 to 103% (mean CV, 3.3%). This method is applicable to determination of α-zearalenol and zearalenone in barley and Job's-tears with satisfactory sensitivity and accuracy.

This publication has 0 references indexed in Scilit: