Arabinosyladenine and arabinosylhypoxanthine metabolism in murine tumor cells

1 January 1970

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 48 (1) , 75-78
https://doi.org/10.1139/o70-013

Abstract

The in vitro metabolism of 9-β-D-arabinofuranosyladenine (Ara-A) in TA3 ascites tumor cells was examined and compared with that of its metabolite 9-β-D-arabinofuranosylhypoxanthine (Ara-H). Some evidence for reamination of Ara-H to Ara-A was obtained. Such reamination may explain its activity as an antiviral agent. Unlike Ara-A, Ara-H was not phosphorylated and it did not inhibit DNA polymerase. Incorporation of radioactivity from Ara-A-¹⁴C or Ara-H-¹⁴C into RNA occurred, but was shown to have proceeded by a cleavage to free base and efficient reutilization of the labeled base.

Keywords

ANTIVIRAL
METABOLITE
TUMOR CELLS
REAMINATION
CLEAVAGE
14C
PHOSPHORYLATED
ARABINOFURANOSYLADENINE
PROCEEDED

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