Regulation of the biosynthesis of acyl analogs of platelet‐activating factor by purinergic agonist in endothelial cells

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Abstract
We have previously shown that platelet‐activating factor (PAF)‐dependent transacetylase (TA) contains three catalytic activities, namely PAF: lysophospholipid TA (TAL), PAF: sphingosine TA (TAS) and PAF acetylhydrolase. It serves as a modifier of PAF actions by producing different lipid signal molecules. The TAL activity is involved in the biosynthesis of acyl analogs of PAF (acyl‐PAF, 1‐acyl‐2‐acetyl‐sn‐glycero‐3‐phosphocholine, acylacetyl‐GPC) in agonist‐stimulated endothelial cells. In the present investigation, we have studied the mechanism(s) by which the TA activity is regulated in ATP‐treated endothelial cells. We have demonstrated that ATP, and thiol‐modifying agents with ATP, specifically regulate only the TAL part of the TA activities.