Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection
- 1 March 1989
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 61 (5) , 432-435
- https://doi.org/10.1021/ac00180a011
Abstract
Naphthalene-2,3-dicarboxaldehyde (NDA) has been investigated as a new derivatizing reagent for the electrochemical detection of tagged amino acids. Gradient elution allowed for the separation of 18 NDA-derivatized amino acids on an open tubular liquid chromatography column in less than 50 min. Gradient elution and electrochemical detection were found to be compatible. A detection limit of 36 amol was obtained for the asparagine-NDA derivative. The usefulness of this technique for quantitation was demonstrated by the analysis of the NDA-tagged hydrolysis from bovine chymotrypsinogen.This publication has 10 references indexed in Scilit:
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