SIMPLE RADIOIMMUNOLOGICAL METHOD FOR DETERMINATION OF RENAL RENIN-ACTIVITY IN RAT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (5) , 1112-1119
Abstract
A RIA [radioimmunoassay] method for the measurement of RRA [renal renin activity] is described, based upon the incubation of 2 dilutions of kidney extract with a fixed amount of EDTA-anticoagulated anephric rat plasma. The RIA of the generated angiotensin I is carried out with the commercially available NEN Kit. Recovery of angiotensin I added to the incubation mixture was 98%. There were 113 kidneys with a wide range of renin activity (52-1274 ng angiotensin I/mg kidney per h) assayed. The within-assay coefficient of variation was uniform throughout the range, equaling 4.4%. The between-assay variance was practically identical with the within-assay variance. The confidence interval to the mean RRA level of one pair of readings was 5.4% of this mean.

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