Analysis of HLA-DP allelic sequence polymorphism using the in vitro enzymatic DNA amplification of DP-alpha and DP-beta loci.
Open Access
- 1 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 141 (11) , 4024-4030
- https://doi.org/10.4049/jimmunol.141.11.4024
Abstract
Allelic sequence variation of the HLA DP-alpha and DP-beta genes has been analyzed in a panel of 34 DP-typed cell lines. The polymorphic second exon of these genes was specifically amplified in vitro by the polymerase chain reaction method, using the thermostable DNA polymerase of Thermus, aquaticus. The analysis of M13 clones containing the amplified DP-beta sequences revealed a total of 14 allelic variants. In general, specific allelic DP-beta sequences were associated with each of the defined DPw1-w6 types, with beta allele subtypes revealed for the DPw2 and DPw4 specificities. An additional six DP-beta alleles which did not correlate with any of the T cell-defined specificities (DP "blanks") were also identified. Only the two previously characterized alleles of DP-alpha were detected. These observations suggest that the T cell-defined DP specificities are determined by polymorphic residues on the beta-chain. The sequence polymorphisms in DP-beta are clustered in a few specific regions, and can be detected using sequence-specific oligonucleotide probes and polymerase chain reaction amplified DNA in a rapid dot-blot format. This approach provides a simple and informative method of DP typing. The DP-beta sequences derived from four DP-typed celiac disease patients were compared with the distribution of DP-beta alleles in control individuals.This publication has 17 references indexed in Scilit:
- HLA-DP and HLA-DO genes in presumptive HLA-identical siblings: structural and functional identification of allelic variation.The Journal of Immunology, 1987
- Isolation and allelic polymorphism of cDNA clones and genomic clones of HLA-DP heavy and light chainsHuman Immunology, 1986
- Increased frequency of HLA‐DPw2 in pauciarticular onset juvenile chronic arthritisTissue Antigens, 1986
- Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic SequencesScience, 1986
- Molecular diversity of HLA-DR4 haplotypes.Proceedings of the National Academy of Sciences, 1986
- Polymorphic restriction endonuclease sites linked to the HLA-DR alpha gene: localization and use as genetic markers of insulin-dependent diabetes.Proceedings of the National Academy of Sciences, 1985
- Human helper T-cell clones that recognize different influenza hemagglutinin determinants are restricted by different HLA-D region epitopesImmunogenetics, 1984
- Mapping SB in relation to HLA and GL01 using cells from first-cousin marriage offspringImmunogenetics, 1983
- Split of HLA‐D into Two Regions α and β by a Recombination between HLA‐D and GLO. I. Study in a Family and Primed Lymphocyte Typing for Determinants Coded by the β RegionTissue Antigens, 1980
- The lymphocyte restimulation system: Evaluation by intra-HLA-D group primingImmunogenetics, 1978