Activation of Chi, a recombinator, by the action of an endonuclease at a distant site.
- 1 April 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (8) , 2310-2313
- https://doi.org/10.1073/pnas.80.8.2310
Abstract
Chi is an Escherichia coli recombinator specific to the recBC pathway. In phage .lambda., its activity is dependent on its orientation with respect to the orientation of the chromosome packaging origin, cos. Chi in an inactive state is achieved by the in vivo action of a restriction endonuclease at a distant site. This result supports a model for Chi activation ion in which a recombination machine enters a DNA duplex at an end and travels until it encounters a Chi properly oriented with respect to the direction of enzyme movement. Events are then initiated that lead to exchange in the neighborhood of Chi.This publication has 33 references indexed in Scilit:
- Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.Proceedings of the National Academy of Sciences, 1977
- A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.1976
- In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.Proceedings of the National Academy of Sciences, 1975
- Rec-mediated recombinational hot spot activity in bacteriophage lambda: III. Chi mutations are site-mutations stimulating Rec-mediated recombinationJournal of Molecular Biology, 1975
- Rec-mediated recombinational hot spot activity in bacteriophage λMolecular Genetics and Genomics, 1975
- Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations.1975
- DNA Nucleotide Sequence Restricted by the RI EndonucleaseProceedings of the National Academy of Sciences, 1972
- Mutants of Escherichia coli with altered deoxyribonucleases: I. Isolation and characterization of mutants for exonuclease IIIJournal of Molecular Biology, 1972
- New mutations in the S cistron of bacteriophage lambda affecting host cell lysisVirology, 1969
- Recombination in bacteriophage λJournal of Molecular Biology, 1968