Structure determination of the bacteriophage MS2
- 1 December 1991
- journal article
- Published by International Union of Crystallography (IUCr) in Acta crystallographica Section B, Structural science, crystal engineering and materials
- Vol. 47 (6) , 949-960
- https://doi.org/10.1107/s0108768191006821
Abstract
The structure of the bacterial virus MS2 has been solved at 3.3 A resolution. Initial phases to 13 A resolution were obtained from a model based on the known coordinates of the plant virus southern bean mosaic virus. These phases were extended in small steps to a resolution of 3.4 A. The phases obtained represented essentially the Babinet opposite of the true structure and were not of a sufficiently good quality to allow an interpretation of the electron density contoured at negative levels. Difference Fourier maps of two heavy-atom derivatives based on these phases were interpretable, and these derivatives were used to calculate isomorphous replacement phases at 8.8 A resolution. Phase extension to 3.3 A resolution led to maps which could be easily interpreted.Keywords
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