An Automated Perifusion System for the Study of Rat Spermatogenesis in Vitroa
- 17 December 1987
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 513 (1) , 146-157
- https://doi.org/10.1111/j.1749-6632.1987.tb25005.x
Abstract
We report details of an automated perifusion system for the study of spermatogenesis in vitro. A potential of this new system is the evaluation of hormone/growth factor effects on the mitotic clonal expansion of spermatogonia and the meiotic and postmeiotic clonal differentiation of spermatogenic cells in coculture with Sertoli cells and in incubated seminiferous tubular segments.Keywords
This publication has 26 references indexed in Scilit:
- Temporal Appearance and Cyclic Behavior of Sertoli Cell-Specific Secretory Proteins during the Development of the Rat Seminiferous Tubule1Biology of Reproduction, 1986
- Fetuin: A serum component associated with rat sertoli and spermatogenic cells in cocultureJournal of Cellular Physiology, 1986
- Chemical messengers between sertoli cells and neighbouring cellsJournal of Steroid Biochemistry, 1983
- The hormonal and cellular conrol of Sertoli cell secretionJournal of Steroid Biochemistry, 1983
- Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcomaBiochemistry, 1982
- Endocrine Signaling and Male ReproductionBiology of Reproduction, 1981
- Association of Nucleoprotein Transitions with Chromatin Changes during Rat SpermatogenesisPublished by Elsevier ,1981
- Spermatogonial stem cells in the albino ratJournal of Anatomy, 1975
- RNA Synthetic Activities of Sertoli Cells in the Mouse TestisBiology of Reproduction, 1974
- The Occurrence of Intercellular Bridges in Groups of Cells Exhibiting Synchronous DifferentiationThe Journal of cell biology, 1959