A 51Cr Microassay Technique for Cell-Mediated Immunity to Viruses

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Abstract
A 51Cr microassay procedure for the in vitro measurement of cell-mediated immunity to rubella virus is described. The technique consists of a lymphocyte target cell interaction employing tissue culture lines chronically infected with rubella virus in which the quantitative release of 51Cr is used as an index of lymphocyte reactivity. Also described in these studies is an apparatus which separates the reacting cells from supernatant fluids and recovers the released 51Cr. Significant release is only observed with lymphocytes from rubella seropositive individuals and the reaction is inhibited by rubella hyperimmune sera. The microassay technique also appears to be applicable for the study of cellular immunity to a wide variety of viral agents.

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