Effects of environmental estrogens on tumor necrosis factor α-mediated apoptosis in MCF-7 cells

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Abstract
Environmental estrogens represent a class of compounds which have been shown to mimic the effects or activity of the naturally occurring ovarian hormone 17β-estradiol. Given the role of 17β-estradiol in cell survival in a number of systems, we wished to determine if environmental estrogens protect MCF-7 cells from apoptosis. Here we demonstrate that the organochlorine pesticides o , p ′ DDT and alachlor, like 17β-estradiol, have the ability to suppress tumor necrosis factor α (TNF)-induced apoptosis in estrogen receptor (ER)-positive MCF-7 breast carcinoma cells. These compounds, however, did not affect TNF-induced apoptosis of the ER-negative MDA-MB-231 cell line. The ability of these compounds to suppress apoptosis in MCF-7 cells was correlated with an ER-dependent increase in Bcl-2 expression. Taken together these results demonstrate that estrogenic organochlorine pesticides like o , p ′ DDT and alachlor may partially mimic the primary endogenous estrogen, 17β-estradiol, and function to suppress apoptosis in ER-responsive cells.