OXOTREMORINE DOES NOT ENHANCE ACETYLCHOLINE RELEASE FROM RAT DIAPHRAGM PREPARATIONS

Abstract
We have reinvestigated the dramatic effect of oxotremorine on acetylcholine release from the rat diaphragm reported by Das, Ganguly & Vedasiromoni (1978), using a rigorous gas chromatographic mass spectrometric/isotope dilution method for identification and measurement of acetylcholine and choline. Oxotremorine (10 μm) causes no significant change in the spontaneous or evoked (1 or 10 Hz) release or in the tissue levels of acetylcholine or choline.