IN VITRO AND IN VIVO LABORATORY EVALUATION OF CEPHALOTHIN, A NEW BROAD SPECTRUM ANTIBIOTIC

Abstract
Boniece , W. S. (Lilly Research Laboratories, Indianapolis, Indiana), W. E. Wick, D. H. Holmes, and C. E. Redman . In vitro and in vivo laboratory evaluation of cephalothin, a new broad-spectrum antibiotic. J. Bacteriol. 84: 1292–1296. 1962.—Cephalothin, a new cephalosporin antibiotic, is the sodium salt of 7-(thiophene-2-acetamido) cephalosporanic acid. The activity of this antibiotic against a variety of gram-positive and gram-negative bacteria is described, as determined by the tube-dilution and disc-plate methods. Correlations of the results of the therapy of experimental infections in mice with in vitro sensitivity to cephalothin are presented. The data from the gram-negative bacterial infection tests suggest the following relationship on a within strain basis: ed 50 = C i ld 50 b i , where the subscript i stands for the various strains.
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